Fluorescence microscope MAGUS Lum 400L

The MAGUS Lum 400L fluorescence microscope can be used for two microscopy techniques: reflected-light fluorescence and transmitted-light brightfield. When equipped with additional accessories, the microscope can also be used for polarized-light research and phase-contrast and darkfield techniques. The microscope can be used for laboratory research and diagnostics in medicine, pharmacology, forensic science, biotechnology, veterinary medicine, etc.

Optics

The trinocular head consists of two optical tubes for observation and a trinocular tube for mounting a digital camera (not included). For comfortable extended observation, the eyepiece tubes can be adjusted by rotating them through 360° to suit the observer's height. They are inclined at 30°.

The five-position nosepiece is inward-facing, allowing the user to see which objective is in the optical path. It is equipped with infinity-corrected plan achromatic objectives: three for brightfield and one for fluorescence. Once all the objectives are mounted on the nosepiece, one position remains available. It is used to adjust the position of the illuminator for reflected-light observations. An optional objective can also be installed to achieve higher magnification.

Illumination

Reflected light: five LEDs rated at 5 W. Each LED corresponds to one of four specific bands: blue (B), green (G), violet (V) and ultraviolet (UV). Turn the ring to select an LED. LED illumination reaches maximum brightness quickly and switches off quickly, making it easy to switch between brightfield and fluorescence. The LEDs do not overheat, require no heat sink and have a service life of 50000 hours before they need to be replaced. LED fluorescence is a safe, easy-to-maintain microscopy technique and is therefore recommended for schools and universities.

Transmitted light is produced by a 3 W LED. The LED service life is 50000 hours. The illumination system also includes a condenser. The condenser has a slot for a darkfield or phase-contrast slider. The slider makes it quick and easy to switch between microscopy techniques without changing the condenser.

Köhler illumination can be set for both transmitted and reflected light. The specimen appears sharp and free of image artifacts. The edges do not darken, and each objective achieves maximum resolution.

Stage and focusing mechanism

The microscope stage is equipped with a removable specimen holder for manual scanning of the specimen. The stage has no rack-and-pinion positioning mechanism, so it requires less workspace. A black plate can be installed on the stage to eliminate unwanted light from the transmitted-light source when observing in reflected light.

The coaxial fine-focus (micrometer) and coarse-focus (macrometer) knobs are located on both sides at the base of the microscope. The coarse-focus mechanism has a locking and tension adjustment knob.

Accessories

The MAGUS Lum 400L microscope is compatible with a wide range of optional accessories that can be used to expand its capabilities. These include eyepieces, objectives, darkfield and phase-contrast devices, polarized-light accessories, digital cameras and calibration slides.

Key features:

• Examination of specimens using reflected light with fluorescence microscopy and transmitted light with brightfield microscopy.
• Infinity-corrected plan achromatic objectives: three brightfield objectives and one fluorescence objective
• Trinocular head with a body that rotates through 360°, trinocular tube for mounting a digital camera
• Four reflected-light LEDs with four different wavelengths: blue (B), green (G), violet (V) and ultraviolet (UV)
• 3 W transmitted-light LED, Köhler illumination for reflected and transmitted light
• Stage without rack-and-pinion positioning mechanism, removable specimen holder
• Wide range of accessories, including phase-contrast, polarized-light and darkfield devices

The package includes:

• Stand with integrated power supply, transmitted-light source, focusing mechanism, stage, condenser mount and nosepiece
• Abbe condenser
• Illuminator for reflected-light observations
• LED lamp housing
• Trinocular head
• Infinity-corrected plan achromatic objective: PL 4x/0.10 WD 19.8 mm
• Infinity-corrected plan achromatic objective: PL 10x/0.25 WD 5.0 mm
• Infinity-corrected plan achromatic fluorescence objective: PL FL 40x/0.85 (spring-loaded) WD 0.42 mm
• Infinity-corrected plan achromatic objective: PL 100x/1.25 (spring-loaded, oil immersion) WD 0.36 mm
• 10x/22 mm eyepiece with long eye relief (2 pcs.)
• Eyecup (2 pcs.)
• UV shield
• Filter slider
• Black protective plate for installation under the stage
• C-mount adapter
• Color filters (4 pcs.)
• Bottle of immersion oil
• Power supply for reflected-light illuminator
• Power cord
• Power cord for reflected-light illuminator
• Power cord for the reflected-light illuminator power supply
• Dust cover
• User manual and warranty certificate

Available on request:

• 10x/22 mm eyepiece with graticule
• 12,5x/14 mm eyepiece (2 pcs.)
• 15x/15 mm eyepiece (2 pcs.)
• 20x/12 mm eyepiece (2 pcs.)
• 25x/9 mm eyepiece (2 pcs.)
• Infinity-corrected plan achromatic fluorescence objective: PL FL 10x/0.35 WD 2.37 mm
• Infinity-corrected plan achromatic objective: PL 20х/0.40 WD 8.8 mm
• Infinity-corrected plan achromatic objective: PL 60x/0.80 ∞/0.17 WD 0.46 mm
• Phase-contrast device
• Darkfield condenser with N.A. 0.9
• Oil darkfield condenser with N.A. 1.36–1.25
• Darkfield slider
• Polarizer
• Digital camera
• Calibration slide
• LCD monitor