MAGUS Lum 400 Fluorescence Microscope
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The MAGUS Lum 400 fluorescence microscope is designed for diagnostics and research and can be used for fluorescence (reflected light) and brightfield (transmitted light) microscopy methods. With additional instrumentation it is possible to use polarization, darkfield and phase contrast techniques. Areas of application of the fluorescence microscope: DNA analysis, pathogen study, health and epidemiological surveillance, forensic expertise, etc.
Optical
It is a trinocular microscope: the binocular head is tilted at 30° and the digital camera (not included) is installed on the trinocular tube. The eyepiece tubes are 360° rotatable, so it is easy to adjust them to suit the user's height.
Five objectives can be installed in the nosepiece. Four infinitely planachromatic objectives are included. One of them is fluorescent with 40x magnification and is designed for fluorescence observations. 4x, 10x, and 100x objectives are used for the brightfield microscopy technique. An additional objective lens can be mounted in the available housing of the nosepiece to achieve higher magnification within the magnification range of the microscope.
The nosepiece is oriented towards the inside of the instrument, so that the user can see which objective is inserted in the optical path.
Lighting
The reflected light source is a 100W mercury vapor lamp. It is located in the lamp housing which offers efficient heat dissipation - the lamp does not overheat even after long hours of operation. The lamp can be centred on three axes to provide optimal illumination of the work area. If it needs to be replaced, simply remove it and install it. The mercury vapor lamp is suitable for fluorescent pigments because they have distinct peaks. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G).
Transmitted light source: 30 W halogen lamp. It has enough intensity to be able to work with any lens, regardless of magnification. Thanks to the color temperature of the illumination, even long-lasting observations are not straining the eyes. The lighting system also includes a condenser. There is a housing for a slider (contrast phase or darkfield).
In transmitted and reflected light, the Köhler lighting method can be set. With this lighting method, the edges do not darken, the sample is clearly visible, and the image has no artifacts.
Stage and focusing mechanism
The microscope is equipped with an ergonomic stage without a positioning rack. For observations in reflected light, a black plate should be placed on the stage, so as to reduce any unwanted light. The sample plate is removable if necessary by loosening two screws. The focus knobs are coaxial and are located on either side of the microscope. The coarse focus mechanism is equipped with fixing and tension adjustment knob, and the fine focus is for sharpness adjustment.
Fixings
The MAGUS Lum 400 microscope can be equipped with additional accessories: digital camera, phase contrast device, polarization device, darkfield condenser, eyepieces and objectives, phase contrast and darkfield sliders. If you want to measure objects, you can equip the microscope with a calibration slide.
Key features:
- Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
- Other search methods with optional accessories: phase contrast, simple polarization, darkfield
- Trinocular head with trinocular tube for the installation of a digital camera 360° rotatable eyepiece tubes
- Infinity planachromatic objectives, including a fluorescent objective
- Reflected light source: 100W mercury vapor lamp, transmitted light source: 30W halogen lamp
- Köhler Illumination Method in Transmitted and Reflected Light
- Fluorescence filters: ultraviolet (UV), violet (V), blue (B), green (G)
- Ergonomic stage with a removable sample holder and no positioning rack
The package contains:
| Brand | MAGUS |
| Guarantee | 5 лет |
| EAN | 5905555018140 |
| Package size (LxWxH) | 45x30x96 cm |
| Shipping Weight | 16.5 kg |
| Type | Biological, Light/Optical |
| Head | Trinocular |
| Seat tube | Gemel cylinder head (Siedentopf type, with 360° rotation) |
| Cylinder head tilt angle | 30° |
| Magnification, x | 40–1000 in basic configuration (*optional: 40–1250/1500/2000/2500) |
| Magnification range | 800x to 1280x |
| Eyepiece tube diameter, mm | 30 |
| Eyepieces | 10х/22 mm, eye relief: 10 mm (*optional: 10x/22 mm with scale, 12.5x/14 15x/15 20x/12 25x/9) |
| Objectives | Infinity planachromatic and fluorescent lenses: PL 4x/0.10, PL 10x/0.25, PL FL 40x/0.85, PL 100x/1.25 (oil) Parfocal distance 45 mm (*optional: PL FL 10x/0.35, PL 20х/0.40, PL 60x/0.80 ∞/0.17) |
| Nosepiece Revolver | for 5 objectives |
| Working distance, mm | 19.8 (4x) 5.0 (10x) 0.42 (FL 40x) 0.36 (100x) 2.37 (FL 10х) ) 8.8 (20х) 0.46 (60х) |
| Interpupillary distance, mm | 48 — 75 |
| Coffee table, mm | 180x150 mm |
| Range of motion of the stage, mm | 75/50 |
| Coffee table features | Two-axis mechanical sideshifter, without positioning rack |
| Capacitor | Abbe condenser, 1.25 N.A. , adjustable centering, adjustable height, adjustable aperture diaphragm, with slot for darkfield slider and phase contrast slider, dovetail mount |
| Diaphragm | adjustable aperture diaphragm, field diaphragm with adjustable iris |
| Fire | Coaxial, coarse focus (21 mm, 39.8 mm/rev, with fixing knob and micrometer screw tension adjustment knob) and fine focus (0.002 mm micrometer screw) |
| Lighting | halogen, fluorescent |
| Adjusting the brightness | Yes |
| Feeding | AC mains, 85–265 V, 50/60 Hz |
| Light source type | reflected light: 100 W mercury vapour lamp transmitted light: 12 V, 30 W halogen lamp |
| Optical filters | Yes |
| Operating Temperature Range, °C | 5 — 35 |
| Possibility of connecting additional equipment | Phase contrast devices (condenser and objectives), darkfield capacitor (dry or oil immersion), polarization instruments (polarizer and analyzer), darkfield slider |
| User level | Experienced users, professionals |
| Assembly and installation difficulty level | complicated |
| Fluorescent module | Filters: Ultraviolet (UV), Violet (V), Blue (B), Green (G) |
| Fluorescence filter: filter type/excitation wavelength/dichroic mirror/emission wavelength | ultraviolet (UV), 320–380 nm/425 nm/435 nm violet (V) 380–415 nm/455 nm/475 nm blue (B) 450–490 nm/505 nm/515 nm green (G) 495–555 nm/585 nm/595 nm |
| Application | laboratory/medical |
| Lighting position | double |
| Research method | brightfield, fluorescence |
| Set with case/case/bag | Dust cover |
