The CHW Ag Test (Canine Heartworm Ag) is a rapid immunochromatographic test used for the qualitative detection of antigens in dog blood, serum or plasma.
Principle of operation:
The lateral-flow immunochromatographic test has a test window. The test window has a T (test) zone and a C (control) zone that are invisible before the sample is added. When the sample is applied to the well, the liquid flows laterally across the surface of the test strip. If the sample contains a sufficient amount of antigens, a visible band will appear in the T zone. The C band, which acts as a procedural control, should always appear after a sample is applied and indicates a valid result.
Kit components:
- 10 aluminum foil pouches, each containing one cassette, one pipette and one desiccant
- 1 diluent container
- 1 instruction manual
Sample preparation:
Collect whole blood, serum or plasma in accordance with standard laboratory procedures.
For serum or plasma samples: centrifuge the dog blood sample to obtain serum or plasma; use the serum or plasma directly for the analysis.
Note: The use of serum or plasma is recommended to increase the test's sensitivity.
Test procedure:
Open the aluminum foil pouch, remove the test cassette and place it on a clean, flat surface.
Use the pipette provided to transfer 1 drop of sample into the well of the test cassette. During this operation, ensure that no air bubbles form inside the pipette.
When the sample has been completely absorbed, add 3 drops of diluent (80 μL) into the well.
Read the results within 5-10 minutes. Results are not accurate after 10 minutes.
Interpretation of results:
- Positive test: two lines are visible in the cassette reaction area (both the C and T lines).
- Negative test: only one line is visible in the area (C).
- Invalid test: if no control line (C) appears after the test, the result is considered invalid. The presence or absence of the (T) line cannot be taken into account. This may occur if the test procedure was not followed correctly or if a test past its expiry date was used; in this case, repeat the test with another package.
The presence of a line next to the letter (C) indicates the control line and confirms that the test was performed correctly.
The (C) line is not a reference line and may have a different intensity from the (T) line.
Test storage:
Store the tests at room temperature (4 ~ 30°C). Do not refrigerate.
Do not store in direct sunlight. The test is valid until the expiry date printed on the package.